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Posted: 19-Nov-24
Location: Los Angeles, California
Categories:
Internal Number: 144540341
Under supervision of the PI and in collaboration with post-doctoral Fellows, the Staff Research Associate II (SRA II) will drive a study that involves molecular biology experiments on a research project related to the identification of asymmetrically-expressed genes in the developing human brain. Job duties include: cutting, mounting, and staining thin brain tissue sections, performing in situ hybridization experiments on mounted tissue sections, maintaining an inventory of brain sections for making tissue slides, and quantification and characterization of gene expression patterns. You will interpret the results, troubleshoot experiments, modify hypothesis, and plan new results based on these interpretations. You will provide technical support in the lab, including technical assistance to other lab researchers in experimental procedures and use of lab equipment and computer programs, assist in training and supervision of lab assistants and graduate students and provide assistance to researchers from collaborating labs in relation to in situ hybridization experiments. The SRA II will provide some laboratory maintenance support including ordering reagents and supplies and preparing buffers, solutions, and chemicals for lab experiments. You will also be responsible for ordering radiological agents and assisting with maintaining the radiation usage log, and assisting with disposal of biological, chemical and radiological waste. Other activities include preparation of figures, charts, and graphs for grant applications, presentations and publications. You will have heavy involvement in writing abstracts and manuscripts for publication.
Salary Range:$28.88 - $46.46/Hourly
Required:
- Demonstrated knowledge of basic, standard laboratory equipment, including Speed Vac, vacuum, centrifuge, waterbath, incubator, and cryostat.
- Good communication skills and ability to work well with multiple individuals.
- Ability to work on several projects simultaneously and to switch focus as needed.
- Ability to learn new laboratory techniques and new lab equipment, as needed.
- Ability to keep accurate, detailed, organized laboratory records.
- Skill in performing repetitive work according to set procedures.
- Demonstrated knowledge of chemistry, buffer solutions, PH, and fixatives for lab experiments.
- Knowledge of laboratory safety procedures for handling chemical, radiological, and biohazard waste.
- Working knowledge of neuroscience discipline and anatomy to recognize areas in tissue.
- Skill in thin sectioning of frozen brain tissue.
- Working knowledge of various tissue staining protocols, including hematoxylin & eosin and cresyl violet.
- Working knowledge of special laboratory techniques, including in situ hybridization, genotyping, PCR, and sequencing.
- Working knowledge of specialized laboratory equipment, including PCR machine, scanner, sequencer, Digital camera and microscopes.
- Working knowledge of and skill in using both Macintosh and PC operating systems.
- Skill in using various computer softwares, such as ImageQuant, NIH Image, Spot Advanced, Adobe Photoshop, Corel Draw, and Excel.
Preferred:
- General knowledge of University procedures for ordering laboratory supplies.
- Willingness to work occasional overtime in order to meet deadlines related to grants applications, conferences, and publications.
- M.S. degree in a biological or chemical science.
